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Microencapsulation with pea-protein improves probiotic survival

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August 27, 2025
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Microencapsulation with pea-protein improves probiotic survival



The researchers on the microencapsulation firm additionally discovered that the pea-protein protected Bifidobacterium probiotics. The paper is printed within the Worldwide Journal of Meals Science and Know-how.

For probiotics to be efficient, they need to survive the cruel situations within the digestive tract, resembling excessive pH and digestive enzymes which will cut back their viability.

“The pea-protein capsule stored most cells alive by means of digestion, which makes delivering the CFU you declare much more dependable,” Dr Gonzalo Saiz-Gonzalo, who led the AnaBio analysis group, instructed NutraIngredients.

“Pea protein is plant-based and non-dairy,” he added. “It kinds a safety, buffering acid after which opens up within the intestine. That’s the reason you get safety and managed launch with out further coatings. That’s arduous to match with alginates or dairy proteins.”

Advantages of pea-protein encapsulation

There are a number of encapsulating brokers that producers might use to enhance the bioavailability of probiotic dietary supplements.

Pea protein is a food-grade, typically acknowledged as protected (GRAS) ingredient that could be a sustainable various to encapsulants derived from animals, resembling milk proteins and gelatin.

The researchers famous that one of the broadly used probiotic encapsulation strategies is alginate hydrogel beads cross-linked with calcium. Producers additionally use starch or dairy-based programs.

“Nonetheless, alginate and starch usually require mixture with proteins or extra coatings to forestall untimely dissolution (e.g., alginate beads are generally coated with chitosan to withstand abdomen acid),” they wrote, noting that utilizing dairy proteins dangers allergy symptoms and is probably not appropriate for some folks’s diets.

“In distinction, pea protein gives a single-component, plant-based various that gives sturdy safety akin to alginate or dairy-based capsules, whereas additionally assembly vegan and allergen-free preferences.”

Research particulars

The examine employed a simulated in vitro mannequin of gastrointestinal digestion (InfoGest) to judge the survival of Lacticaseibacillus rhamnosus GG microencapsulated in food-grade pea protein isolate in comparison with a non-encapsulated management.

Findings confirmed that the non-encapsulated probiotic misplaced roughly 50% viability throughout gastric digestion, and fewer than 20% was alive after subsequent intestinal digestion.

In distinction, the pea-protein encapsulated L. rhamnosus GG maintained 80% to 100% viability all through the simulated digestive course of, demonstrating higher survival than the management even at larger gastric pH.

“The bodily barrier fashioned by the pea protein matrix not solely protects the cells from direct publicity to low pH and proteolytic enzymes but in addition gives a buffering impact towards speedy pH fluctuations”, the examine concluded..

The researchers additionally performed complementary in vitro digestion assays utilizing Bifidobacterium animalis subsp. lactis HN019. They discovered that the encapsulated Bifidobacterium lactis “maintained excessive viability throughout each gastric and intestinal digestion phases, whereas the non-encapsulated B. lactis exhibited a drastic lack of viability, with no important restoration of reside cells by the tip of the intestinal part,” underscoring the broader applicability of pea-protein encapsulation.

Supply: Worldwide Journal of Meals Science and Know-how, 60 (2), vvaf154. doi: 10.1093/ijfood/vvaf154, “Pea protein microencapsulation improves probiotic survival throughout gastrointestinal digestion”. Authors: G. Saiz-Gonzalo et al.



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